FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_M1_uncut_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_M1_uncut_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13700083
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411780.30056752210917265No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358820.2619108220001295No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185150.13514516663877146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT505050.067.906841
CTTTGTG1215750.050.5623551
TTTGTGT1225450.050.2565272
TGTGTTT1442850.044.5494424
TTGTGTT1510200.042.5217973
GTGTTTG2324300.037.1719931
GTTTGAT1209650.034.6752433
TTTGATT832350.030.2440034
GATTTTT416250.029.3808147
TGTTTGA2959300.029.2186342
GTTTGAC593200.029.209433
GGTAACT129150.028.8766021
TTTGATC311550.028.7987464
TGATTCC134700.028.5268276
TTGATTT537500.027.6944715
TGATTTT477700.026.4583156
TTTGACG160050.026.412154
TTGATCG94800.026.404315
CCTTGTG50500.025.4005551
TAACTTT142750.025.2875563
Produced by FastQC (version 0.11.5)