FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_M1_uncut_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_M1_uncut_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13494215
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347110.25722874579958893No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323500.2397323593851143No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176760.13098946474470727No Hit
GTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA152950.11334486667064368No Hit
CTTTGTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT146270.10839459724037301No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC135650.10052455811620016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT607900.066.700461
TTTGTGT1429900.049.518012
CTTTGTG1432700.049.330731
TGTGTTT1627750.045.625934
TTGTGTT1728800.042.8519023
GGTAACT151450.032.779661
CCTTGTG60100.032.171951
GTGTTTG2408850.031.7738761
CTTGTGT101650.030.3727822
GTTTGAT1186850.029.818963
GATTTTT417600.027.8323886
TAACTTT186350.025.9727863
TTTGATT769650.025.9320324
TTGAGGG189350.025.7602429
TTGATCG93150.025.750495
TTTGATC301100.025.6024514
TGAGTCG78650.025.3645735
TTGAGTC241250.025.2009224
TGATTTT472650.025.1635345
GTTTGAC720400.024.8812967
Produced by FastQC (version 0.11.5)