FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_M1_4h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_M1_4h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12489130
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316290.25325222813758846No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260290.20841323615015617No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141700.11345866365391345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT457250.063.1947561
CTTTGTG1081700.049.895321
TTTGTGT1091850.049.4937632
TGTGTTT1362800.041.3362734
TTGTGTT1448350.038.8980873
GTGTTTG2209500.037.5201761
GTTTGAT1083150.034.7178273
TAACTTT151800.031.4466783
TTTGATT666350.031.2996714
TTTGATC278000.031.249834
TTGATCG84150.030.8755995
GTTTGAC595600.030.1850363
GATTTTT315400.030.0865617
GGTAACT166450.029.9990311
TGTTTGA2774100.029.9127622
TTGATTT424600.027.5192995
TGATTCC112000.027.0149176
TTTGACG156150.027.0115644
TGATTTT368400.026.8179726
TTGATGC116250.025.7821185
Produced by FastQC (version 0.11.5)