FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_M1_4h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_M1_4h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14015870
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271680.19383741430250137No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252000.1797961881781152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT471150.065.4199451
TTTGTGT1192350.052.7240752
CTTTGTG1204550.052.155071
TGTGTTT1423050.046.1039434
TTGTGTT1544250.042.4276543
GTGTTTG2140800.032.9118921
GTTTGAT1099900.029.7149333
CCTTGTG51050.028.1971631
GATTTTT303550.028.1654786
CTTGTGT89500.027.2790282
TGAGTCG86200.027.1101995
TTGAGTC237300.026.781114
TTTGAGT377650.026.6764243
TTTGATT667200.026.446794
TTTGATC310400.026.197044
TTGATCG90150.026.185785
TTGAGGG174300.025.3142649
GTTTGAA209400.024.793182
TGATTCC120750.024.781546
GTTTGAC637700.024.6093433
Produced by FastQC (version 0.11.5)