FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_M1_30min_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_M1_30min_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13267731
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348030.26231312648711375No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324970.24493261131085642No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157490.11870153231174194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT462750.067.420751
CTTTGTG1120400.050.74831
TTTGTGT1127200.050.5022
TGTGTTT1328800.044.8763124
TTGTGTT1452250.041.0450443
GTGTTTG2114900.036.292511
GTTTGAT1160600.032.784053
TTTGATT764950.029.2633764
GATTTTT398250.028.9080567
TTTGATC287350.027.9176274
GTTTGAC560600.027.6707863
TGTTTGA2778800.027.6596342
TGATTTT452250.026.1929556
TTGATTT511750.026.1361395
TTGATCG87800.026.1287845
TGAGTCG74050.025.5284615
CTTGTGT91550.025.4738562
TGATTCC126000.025.4448456
TTTGACG148400.025.3484464
CCTTGTG54850.025.0319231
Produced by FastQC (version 0.11.5)