FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_M1_30min_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_M1_30min_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9447216
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222130.23512747035740475No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172810.18292161415595876No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105110.11126029086240856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT415650.069.8574141
TTTGTGT918500.047.446412
CTTTGTG932300.046.6406631
TGTGTTT1047900.043.65874
TTGTGTT1123950.040.6373
GTTTGAT706050.031.0597953
GTGTTTG1519450.030.1809461
GATTTTT235000.028.982127
TTTGATT442550.028.6350334
CCTTGTG41300.027.9520171
CTTGTGT69500.027.4737362
TGATTTT267750.026.2726786
TTGATTT306000.026.18625
GTTTGAC438200.025.5576157
TTGAGGG118400.024.6300669
TTTGATC196550.024.6007824
TTTGACG121300.024.5502348
TTGATCG57150.024.1855285
TGTTTGA1947050.023.5923272
GTTTGAG920750.023.5012457
Produced by FastQC (version 0.11.5)