FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Col-0_uncut_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Col-0_uncut_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11226261
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279220.24872038873851232No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273380.24351830052766454No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138710.12355850269292687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT341850.061.5498241
CTTTGTG863500.052.4033931
TTTGTGT881500.051.4038622
TGTGTTT1076650.043.9256174
TTGTGTT1141100.041.423863
GTGTTTG1702900.036.1497731
GTTTGAT935000.031.91683
GTTTGAC443450.028.0835463
TGTTTGA2199500.028.0060712
TTTGATT623600.027.6708934
GATTTTT325150.027.1394277
TTTGATC234550.026.1834564
TTTGACG117650.025.8375854
GGTAACT119100.024.9700451
TTGATCG73150.024.6739655
TTGATTT421750.024.5736315
TGATTTT374300.024.262626
TAACTTT119150.024.0772443
CCTTGTG47500.022.9033281
TTGAGGG131750.022.6024579
Produced by FastQC (version 0.11.5)