FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Col-0_uncut_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Col-0_uncut_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12088754
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354260.2930492257514712No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300510.24858641345501778No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151240.12510801361331367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT454900.066.934731
TTTGTGT1007150.047.015522
CTTTGTG1013150.046.620531
TGTGTTT1173150.042.6692734
TTGTGTT1242500.040.1731763
GTGTTTG1768000.033.2510221
GTTTGAT965300.032.2882963
GATTTTT360350.029.1273657
TTTGATT647350.028.5783864
TTGATTT454000.027.2643475
TGATTTT401650.027.1637556
CCTTGTG52000.026.1300281
CTTGTGT91200.025.6244182
TGTTTGA2309050.025.4954052
TTGATCG74700.025.1797335
TTTGATC254400.025.148954
GTTTGAC509950.023.6186527
TTTGACG131750.023.1068558
TTGAGGG133200.023.0692529
GGTAACT110900.023.0048791
Produced by FastQC (version 0.11.5)