FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Col-0_4h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Col-0_4h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9297183
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204660.22013119457797053No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162100.17435388762381035No Hit
GTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA95940.10319254767815154No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95160.10235358387589014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT347300.064.728621
TTTGTGT838950.050.394642
CTTTGTG839700.050.2734341
TGTGTTT1022800.042.9838144
TTGTGTT1095100.040.0941283
GTGTTTG1592900.034.6407361
GTTTGAT779300.032.7847253
GATTTTT216450.030.4351087
TTTGATT486000.029.8473014
TGATTCC85900.028.200326
TTTGATC209400.027.2873444
TGTTTGA2038400.027.0861842
TTGATTT298250.027.0735655
GTTTGAC449000.026.9331233
TTGATCG63750.026.8225025
TGATTTT257400.026.5170486
TTTGACG115750.025.8928994
TTGATTG73400.025.3021645
TTGATGC80800.024.9835745
CTTCCGA23000.024.3683724
Produced by FastQC (version 0.11.5)