FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Col-0_4h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Col-0_4h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9966865
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193650.19429379248138706No Hit
GTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA191880.1925179080884511No Hit
GTGTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT170520.1710868964313252No Hit
CTTTGTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT166880.1674347951938749No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156300.15681962181689027No Hit
CTTTGTGTTTGAGCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGT145460.14594358406580205No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC132040.13247896906399353No Hit
GTGTTTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATC122170.12257615609321487No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC120140.12053940732617528No Hit
CTTTGTGTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGT118600.1189942875718694No Hit
GTGTTTGATGCCCGCGTCGCGATAGTAATTCAACCTAGTACGAGAGGAAC108780.10914164082687988No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108290.10865001181414617No Hit
GTGTTTGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATT105700.1060514013182681No Hit
GTGTTTGAGCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGG103290.10363338923523094No Hit
TGTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTG101880.10221870166797684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT676000.060.8565671
GGTAACT206700.052.7507481
CTTTGTG1517300.048.856581
TTTGTGT1520750.048.7912032
TAACTTT235700.044.6762773
TGTGTTT1769400.043.5006834
GTAACTT252300.043.169532
TTGTGTT1800050.042.6964453
CCTTGTG42500.035.6605571
TTGATCG89300.035.1582645
TGATTCC101700.034.654566
TTTGATC318100.033.700864
AACTTTG317250.033.2970854
GTTTGAT977900.032.969953
GTGTTTG2646450.032.0665931
CTTGTGT79400.030.7491512
TTGATGC79800.030.4750585
TGATCGG72500.029.3506346
TTTGATT510000.029.029514
GATTTTT184900.028.948887
Produced by FastQC (version 0.11.5)