FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Col-0_30min_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Col-0_30min_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8864323
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328380.3704513023724429No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165540.18674861013074545No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127140.14342888904206222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT339950.068.743561
TTTGTGT694500.042.1777842
CTTTGTG698100.041.9143561
TGTGTTT831150.036.773634
TTGTGTT862600.035.388833
GTGTTTG1232000.032.341951
GATTTTT278500.031.7885637
GTTTGAT680850.031.7414913
TTGATTT354800.029.0504765
TTTGATT496650.028.9869694
TGATTTT324850.028.1904056
TGTTTGA1533850.025.9782162
ATTTTTT383450.023.0508968
GTTTGAC284650.022.876713
TTTGATC168300.022.1540364
TTGAGGG128050.020.9565149
TTGATCG52050.020.6236175
GTTTGAG720650.020.213417
TTTGACG88700.019.4371788
TTTGAGT231750.017.911918
Produced by FastQC (version 0.11.5)