FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_Col-0_30min_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_Col-0_30min_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10857559
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278970.2569362045373182No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231800.21349181708337941No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134020.1234347425604595No Hit
GTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA114210.10518938925406715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT502950.066.878821
TTTGTGT1125200.047.9946332
CTTTGTG1137700.047.4259451
TGTGTTT1298550.043.7191854
TTGTGTT1364800.041.5140153
GGTAACT116200.032.545211
GTGTTTG1945550.032.4755631
GTTTGAT945900.032.053523
CCTTGTG52750.030.1718861
CTTGTGT86050.028.0422552
TTTGATC253100.028.03814
TTTGATT603250.028.0312714
GATTTTT311850.027.88687
TAACTTT141900.025.7418673
TGTTTGA2475400.025.5714022
GTTTGAC564100.025.4468753
TTGAGGG142700.025.4287789
TTGATTT408050.025.4001055
TTTGACG148150.025.2627548
TGATTCC103850.025.2479846
Produced by FastQC (version 0.11.5)