FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_uncut_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_uncut_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10949717
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303030.27674687848096896No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291740.26643610971863474No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151920.13874331181344687No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC115900.10584748446010066No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC112010.10229488122843722No Hit
GTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA110730.10112590124475362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT489050.066.671131
TTTGTGT1109800.048.7200552
CTTTGTG1112100.048.572861
TGTGTTT1256500.045.0228584
TTGTGTT1353900.041.7593463
GTGTTTG1878400.032.346371
GGTAACT117450.031.7941041
GTTTGAT886500.031.3283963
CCTTGTG46150.030.1628931
CTTGTGT77600.028.9531042
GATTTTT310000.027.7464167
TTTGATT573400.027.2700544
TTTGATC232550.026.9613384
TTGAGGG148050.025.375719
TTGATTT395550.025.3740545
TGATTTT348850.025.3531976
TTGATCG73450.025.221135
TTTGACG147350.025.1114144
TGTTTGA2428150.025.0495432
GTTTGAC545200.024.6293983
Produced by FastQC (version 0.11.5)