FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_uncut_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_uncut_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8135762
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234280.2879631926302662No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133630.1642501341607584No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102430.12590092974696163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT315600.068.883721
CTTTGTG668550.045.03871
TTTGTGT677800.044.541462
TGTGTTT818350.038.4405674
TTGTGTT861800.036.4859473
GTGTTTG1227200.032.731491
GATTTTT251200.032.368777
GTTTGAT657750.031.816893
TTTGATT443750.030.2166714
TTGATTT311800.029.3397885
TGATTTT285200.029.0784666
TGTTTGA1541050.026.0972632
GTTTGAC344700.024.1829873
ATTTTTT347100.023.3708938
TTTGATC158850.023.2929424
TTTGACG88200.021.2717194
TAACTTT79500.020.915511
TGATTCC69450.020.791136
TTGATTG65750.020.7330385
TTGATCG50050.020.4987095
Produced by FastQC (version 0.11.5)