FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_4h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_4h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13048491
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGTGTTTGAGCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGT341650.2618310423787701No Hit
ACTTTGTGTTTGAGCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTG236790.18146925954886278No Hit
GTGTTTGATCCCTGGTCGGCATCGTTTATGGTTGAGACTAGGACGGTATC205290.15732853706991867No Hit
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189270.14505125535205565No Hit
GTGTTTGAGCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGG188520.14447647624541413No Hit
TGTTTGAGCCCGGTATTGTTATTTATTGTCACTACCTCCCCGTGTCAGGA174830.1339848416188508No Hit
CTTTGTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT173430.1329119206197866No Hit
GTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA161960.1241216321488822No Hit
GTGTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTT152490.11686408796235519No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC151460.11607472465590082No Hit
CTTTGTGTTTGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTT143960.11032693358948556No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141620.108533622776764No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC139680.10704686082091792No Hit
GTGTTTGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA130930.10034110457676677No Hit
CTTTGTGTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGT130720.10018016642690715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT885550.070.611291
TTTGTGT1917300.047.2479862
CTTTGTG1938750.046.658771
TGTGTTT2170500.043.6741454
TTGTGTT2275700.041.6136133
CCTTGTG54500.041.0572431
CTTGTGT94050.040.1499942
GGTAACT191450.033.847331
GTTTGAT1123300.031.0785273
GTGTTTG3088300.030.7057915
TGATTCC127150.030.5941246
TTTGATC411000.029.2725494
ACTTGTG54000.028.8566191
TTGATCG102550.028.5772235
GTAACTT224950.028.5263712
TTGAGGG243100.028.4847979
CTTCCGA27300.028.1850974
TTTGATT551200.028.0226044
GATTTTT182950.027.5178187
TTGATGC99150.027.3056565
Produced by FastQC (version 0.11.5)