FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_4h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_4h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10846132
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302380.2787906324577278No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155170.14306482716603486No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114600.10565978728638006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT431250.072.333571
CTTTGTG847000.041.3158261
TTTGTGT853150.041.0595932
TGTGTTT1016550.036.0710334
GATTTTT270550.032.862637
TTGTGTT1128850.032.545743
GTTTGAT803600.032.1535533
GTGTTTG1491700.031.3769741
TTTGATT533400.030.364874
TTGATTT355800.029.2490985
TGATTTT317100.029.014126
TGTTTGA1882350.024.9332312
TAACTTT97350.023.8150791
TTTGATC205350.023.5694524
ATTTTTT377700.023.4014848
CTTGTGT67800.022.6983012
CTTCCGA29600.021.9836034
GTTTGAC373950.021.3004883
TGATTCC94000.021.1720166
TGAGTCG50400.020.5448025
Produced by FastQC (version 0.11.5)