FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_30min_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_30min_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11828817
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205300.17355919869248126No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196380.16601829244631985No Hit
CTTTGTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT164250.13885581288475424No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGC153650.12989464626936065No Hit
GTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA139160.11764490058473302No Hit
CTTTGTGTTTGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTT125950.10647725803856802No Hit
GTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGC121760.10293506104625677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT612300.069.723131
TTTGTGT1399700.049.6038442
CTTTGTG1428750.048.56131
TGTGTTT1577250.045.891614
TTGTGTT1707750.042.432033
CCTTGTG43600.035.5226861
CTTGTGT80100.033.267672
GTGTTTG2224350.032.5243155
GTTTGAT952500.028.1997343
TTGAGGG176250.028.1057939
TTTGACG179700.027.5926288
TGATTCC104950.026.9288256
TGAGTCG82350.026.6477935
TTTGATT541650.026.2373644
GTTTGAC707250.026.1092247
TTTGATC263750.026.0947594
GATTTTT229100.025.8305477
TTGAGCG96950.025.5719229
TTTGAGT400900.025.259723
TTGAGTC243750.025.157014
Produced by FastQC (version 0.11.5)