FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_30min_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_30min_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11958144
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291650.24389236322961155No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285460.2387159746529227No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134380.11237529837406207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT411700.066.7677841
TTTGTGT931100.048.4074022
CTTTGTG941450.047.8337751
TGTGTTT1096000.043.242034
TTGTGTT1194550.039.5912443
GTGTTTG1641650.032.7072561
GTTTGAT957700.028.9491063
GATTTTT355450.027.0862186
TTTGATT639650.026.6578564
CTTGTGT84400.024.7626952
TTGATTT446800.024.6737465
TGATTTT408250.024.3276986
TGTTTGA2212700.024.2960032
TTTGAGT281500.023.3693333
GTTTGAC440250.023.1636833
TTTGATC234150.023.0236854
TTGAGGG125350.022.6964269
CCTTGTG52350.022.5970651
TGAGTCG61600.022.4380665
TTGATCG70650.022.387485
Produced by FastQC (version 0.11.5)