FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_24h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_24h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12657633
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324470.25634334634287465No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291910.23061973751332498No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165660.1308775503287226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT585900.067.776421
TTTGTGT1252100.046.4859282
CTTTGTG1257500.046.1988071
TGTGTTT1432350.042.7873654
TTGTGTT1537800.039.803623
GTGTTTG2053300.029.9245931
GTTTGAT1002950.029.589143
CCTTGTG56200.028.3175891
GATTTTT341200.027.311697
CTTGTGT99650.026.7404162
TTTGATT643950.026.4208934
GGTAACT127550.026.3694211
TTGAGGG159900.025.5451589
TTGATTT438000.024.8227065
GTTTGAC578550.024.5218327
TTTGATC266000.024.5167544
TGATTTT392250.024.472646
TTGATCG88650.024.3250985
GTTTGAG1183700.023.9707817
TGTTTGA2620450.023.4716132
Produced by FastQC (version 0.11.5)