FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_24h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_24h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12787383
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314950.2462974636796286No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271210.21209187212113692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT562100.073.3112951
TTTGTGT1215950.046.6174772
CTTTGTG1215650.046.4936831
TGTGTTT1396550.042.7750474
TTGTGTT1541850.038.645613
GTGTTTG2105100.033.098171
GTTTGAT1106050.031.8940963
CCTTGTG45750.030.8411391
CTTGTGT85650.030.168242
GATTTTT317200.029.7361977
TTTGATT697000.029.2207894
TGAGTCG84950.027.9562055
TGATTCC117150.027.529556
TTGATCG77500.027.0276625
TTGATTT430650.026.8452685
TTTGATC279500.026.5781574
TTGAGTC223700.026.392174
TTTGAGT361000.026.222363
TGATTTT379350.025.7802946
TTGATGC114800.025.1141475
Produced by FastQC (version 0.11.5)