FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_14h_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_14h_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13091406
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277840.21223083296018777No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202560.15472746013682565No Hit
CTTTGTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCAT153470.11722957793838186No Hit
GTGTTTGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCA150390.11487688946473741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT575250.068.215781
TTTGTGT1258400.047.7064932
CTTTGTG1292250.046.3990061
TGTGTTT1455900.043.3613664
TTGTGTT1546150.040.812053
CTTGTGT89750.030.801512
GTGTTTG2127400.030.7925071
GTTTGAT976400.030.6618273
CCTTGTG48700.030.0457611
GATTTTT287750.029.6772047
TTTGATT602500.027.742084
CTTCCGA28150.026.6597144
TTGATTT388150.026.5301575
TGATTTT342300.026.1290476
TTGATCG83400.025.9704175
TTGAGGG172350.025.8212329
TTTGACG167750.025.6232788
TTTGATC262100.025.2985274
GTTTGAC647300.024.6463747
TGTTTGA2681650.024.4557322
Produced by FastQC (version 0.11.5)