FastQCFastQC Report
Thu 14 Nov 2019
HJ777BGXC_n01_CNQX_14h_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ777BGXC_n01_CNQX_14h_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12725410
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277570.21812263809181787No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277100.2177532983220187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGT449700.071.385721
TTTGTGT1035100.049.3016662
CTTTGTG1046850.048.709871
TGTGTTT1190950.045.0582124
TTGTGTT1342250.039.9299743
GTGTTTG1787350.032.9284971
GTTTGAT1007100.029.8208453
CCTTGTG47350.029.097681
CTTGTGT80400.028.0617942
TTTGATT633200.027.3865184
GATTTTT307850.027.3536367
TTTGAGT314450.026.5241993
TGAGTCG71600.025.672555
TTGAGTC195750.025.3802474
TTGATTT410150.025.222395
TTTGATC261600.025.1102144
TTGATCG74300.024.8035565
GTTTGAA184150.024.7615262
TTGAGGG145150.024.4754149
TGATTTT359250.024.3007566
Produced by FastQC (version 0.11.5)