FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21591605
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3081071.4269759010504315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG37950.018.40131250-51
AAAGGGG118000.016.87420580-81
GGGGAGA86150.015.2784064
TCGGAAG143400.014.51178553
GGGGGGA159850.014.3828722
ATCGGAA147800.014.1092182
CGGAAGA149750.013.9617374
TCGTGTA74950.013.73214-15
GATCGGA147700.013.5375651
CGTGTAT12650.012.96510814-15
AAAAAGG181550.012.8252678-79
ATTACGA15000.012.66606154-55
GTAGGGA74650.012.57467418-19
CGGGGAT21750.012.2283461
TATAGGG21550.011.57473248-49
AAAAGGG127750.011.48076680-81
AGCGTCG91000.011.46641210-11
AGGGGGG193950.011.36669982-83
AAGGGGG123850.011.15155482-83
GTGTAGG82600.011.13563716-17
Produced by FastQC (version 0.11.5)