FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24272938
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG140500.020.85931650-51
AAAGGGG354550.017.26939680-81
GGGGAAT184350.015.37823662-63
GGGGGGA408150.014.0353052
AAAAAGG484750.013.36589278-79
TATAGGG76750.013.18341248-49
GGGGGAG401600.013.0352643
GGGAAAA363850.012.67845622-23
AGGGGGG518700.012.53193982-83
ATAGGGG88600.012.30482848-49
GGGGGGT446000.011.91331914-15
GAATTAA163950.011.78904166-67
GGAATTA138200.011.528564-65
GGGGTTT251400.011.33914140-41
GGGAATT158050.010.74162264-65
AAAAGGG371300.010.36502478-79
AAGGGGG365000.010.28347882-83
GGGTTGG211500.010.08872316-17
TTAGGGG119400.09.90653148-49
GGGGGAA368050.09.81868162-63
Produced by FastQC (version 0.11.5)