FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24986147
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG141250.023.01796750-51
AAAGGGG382850.017.23886380-81
TATAGGG85300.016.95760748-49
GGGGGGA434150.015.9301732
GGGGGGT369850.015.76749514-15
GGGGAAT196500.015.59908762-63
GGGGGAG427300.014.2747763
AAAAAGG490150.014.19690378-79
ATAGGGG103200.014.01631748-49
GGGAAAA384500.013.98717222-23
TTAGGGG122150.013.43633748-49
TTATAGG101500.013.29246646-47
GGGGGTT206000.013.15077814-15
AGGGGGG530500.013.080585582-83
TTTAGGG140400.012.9416848-49
GAATTAA162750.012.32816966-67
AAAAGGG382600.011.7834580-81
GGGTTGG216600.011.65082716-17
AAGGGGG396000.011.3964882-83
GGAATTA143400.011.25938964-65
Produced by FastQC (version 0.11.5)