FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24641312
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG139950.022.19706550-51
AAAGGGG363400.016.98635580-81
GGGGAAT184650.014.76146562-63
TATAGGG79050.014.36254248-49
AAAAAGG478750.013.6675678-79
GGGGGGA411450.013.2413892
AGGGGGG515600.012.91138782-83
ATAGGGG93500.012.65094648-49
GGGAAAA355950.012.54615622-23
GGGGGGT458800.012.01116214-15
GGGGGAG418650.011.7556123
AAGGGGG365350.011.56705180-81
AAAAGGG368550.011.56348178-79
GAATTAA160400.010.58416666-67
GGGAATA129600.010.07635464-65
CGCGAAT9459.094947E-1110.0549251
GGAATTA147400.09.98711164-65
TTAGGGG119100.09.89179648-49
GGGGGAA366200.09.83567462-63
GGGGTTT251900.09.80785240-41
Produced by FastQC (version 0.11.5)