FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26461884
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG141550.019.98254450-51
AAAGGGG367200.018.05138880-81
CGCGAAA14550.015.6742091
CGGGGAT97250.014.2170911
GGGGAAT188400.014.07708762-63
AGGGGGG551850.013.05244782-83
AAAAAGG548350.012.99760778-79
GGGGGGA451350.012.6818932
GGGGGAG409700.012.661553
GGGGGGT461350.012.61963614-15
GGGAAAA386000.011.19396122-23
AAGGGGG395350.011.193480-81
ATAGGGG113350.011.10575248-49
AAAAGGG431900.010.53778878-79
ACGATCG19050.010.4733233
CGCGGGT57800.010.1929811
GGGAATT164250.010.19143564-65
GGAATTA142650.010.18664764-65
CGGGATT62150.010.0911391
GAATTAA176800.010.08589866-67
Produced by FastQC (version 0.11.5)