FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26283038
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG154650.023.20439750-51
AAAGGGG383250.019.1538580-81
GGGGAAT205550.016.12512462-63
GGGGGAG412150.015.75563053
GGGGGGA470100.015.3477472
GGGGGGT441700.014.940557514-15
AGGGGGG555250.014.15685982-83
AAAAAGG573050.013.76328278-79
GGGAAAA408550.013.5412722-23
CGCGAAA15950.013.1060931
TTTAGGG151600.012.3772548-49
CGGGGAT96800.012.1227981
GGAATTA156650.012.00450964-65
GGGGAGA316300.011.790944
GAATTAA191100.011.7790166-67
AAGGGGG400550.011.37403482-83
GGGAATT174350.011.33054964-65
GGGGGAA399200.011.12803362-63
TTAGGGG164700.010.97456648-49
GGGTTGG231900.010.72838816-17
Produced by FastQC (version 0.11.5)