FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25473530
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG135450.019.0940250-51
AAAGGGG352350.015.09360880-81
AAGGGGG354900.014.6038480-81
AAAAGGG374100.013.93711578-79
AGGGGGG479950.012.93068982-83
CGCGGGT50850.012.4270961
CGCGTAT6201.6249032E-811.4949891
AAAAAGG495750.011.33131578-79
GGGGAAT184100.010.29210662-63
GGGGGGT490950.010.15932114-15
TTAGGGG140100.09.697288548-49
TAAAAAA745750.09.5490170-71
AAAAAAG688450.09.28084476-77
TTTAGGG140350.09.27386148-49
GGGGGGA434250.09.07656960-61
CCGGGAT74850.08.8233191
GGGAAAA331300.08.768867522-23
CGACCTA14400.08.5779274
GGGGGAG429250.08.4438163
CGGGGAT87150.08.0686961
Produced by FastQC (version 0.11.5)