FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26180516
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG131800.019.73121550-51
AAAGGGG334850.015.26492180-81
GGGGGGA372700.013.5606932
GGGGGGT344800.013.25300914-15
GGGGAAT183350.012.85905362-63
AGGGGGG440000.012.38303582-83
GGGGGAG378000.012.1399163
CGCGGGT54550.011.9328261
TATAGGG100250.011.60944948-49
ATAGGGG102750.011.39633148-49
AAAAAGG486000.011.22119778-79
CGCGATT10250.011.1251241
GGGAAAA367350.011.0768522-23
CGGGGAT91700.010.0001261
AAGGGGG333250.09.75282482-83
TTTAGGG144150.09.45795448-49
GGAATTA141600.09.2226764-65
TTATAGG115300.09.14690946-47
AAAAGGG360650.09.11726780-81
GGGGGAA350900.09.100862-63
Produced by FastQC (version 0.11.5)