FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26533433
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG209950.026.43612750-51
AAAGGGG506000.021.41412480-81
GGGGAAT232750.019.77003162-63
TATAGGG122100.017.85747748-49
AAAAAGG641450.017.6257978-79
ATAGGGG137650.017.2723348-49
GGAATTA166100.016.06768864-65
GGGGGGT615400.015.91697914-15
GAATTAA205650.015.82988566-67
GGGAATT190050.015.41714864-65
GGGAAAA439250.015.40171922-23
AAAAGGG533450.015.32820878-79
AAGGGGG531250.015.26149180-81
GGGGGAG526250.015.213
AGGGGGG796300.014.80578782-83
GGGGGGA634900.013.9755072
TAAAAAA863650.013.85159170-71
TTTAGGG153450.013.11020848-49
GGGGGAA491050.012.43622362-63
TTAGGGG168050.012.381083548-49
Produced by FastQC (version 0.11.5)