FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25228719
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG192800.022.4804350-51
AAAGGGG482700.021.92165880-81
GGGGAAT225850.018.42916162-63
AAAAAGG618950.017.8213978-79
AAAAGGG508250.017.17648178-79
AAGGGGG507600.017.09050880-81
AGGGGGG788150.014.86859782-83
GGGAAAA405500.014.861822-23
GGAATTA161500.014.52577864-65
GGGGGAG514000.014.4911573
GGGAATT183850.014.42595264-65
GAATTAA197050.014.13468166-67
TAAAAAA873200.013.68909370-71
GGGGGGA625550.013.2199872
TATAGGG99150.012.74441148-49
GGGGTTT319550.012.66778140-41
AAAAAAG859250.012.45384976-77
GGGGGAT208800.012.32657862-63
ATAGGGG113000.012.14927348-49
GGGGGAA460950.011.99148362-63
Produced by FastQC (version 0.11.5)