FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34102660
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG267750.027.213350-51
AAAGGGG651950.022.21036780-81
GGGGAAT321500.020.22844962-63
TATAGGG157200.019.08278848-49
AAAAAGG842250.017.99310378-79
GGGGGAG661650.017.992283
GGGGGGT704700.017.8275114-15
TTTAGGG229700.017.68178648-49
GGGAAAA599200.017.56982222-23
ATAGGGG174100.016.97120348-49
GGAATTA219400.016.47185964-65
TTAGGGG230250.016.21600748-49
GAATTAA273450.016.12527566-67
GGGGGGA824700.016.0635682
TTATAGG175650.015.76747346-47
AGGGGGG993950.015.48054982-83
GGGAATT257200.015.02038964-65
GGGGGTT359350.014.60047814-15
GGGTTGG345200.014.220716-17
GGGGAGA494650.013.9651124
Produced by FastQC (version 0.11.5)