FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27878620
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG194350.023.48662650-51
AAAGGGG483800.020.38494180-81
GGGGAAT243750.017.6016762-63
AAAAAGG633100.016.35065378-79
GGGAAAA438600.015.01282722-23
AAAAGGG499950.014.78336978-79
GGAATTA164650.014.65161764-65
AGGGGGG740150.014.59173582-83
TATAGGG106450.014.45874548-49
AAGGGGG505050.014.41744180-81
GGGGGAG522900.014.1261923
ATAGGGG122100.013.9477748-49
GAATTAA213350.013.93401266-67
GGGAATT199000.013.48276564-65
GGGGGGA611450.013.4236682
GGGGGGT702350.012.42714114-15
TAAAAAA874900.012.38941470-71
GGGGTTT316750.012.25467540-41
GGGAATA150500.011.62745364-65
GGGTTGG279900.011.52002916-17
Produced by FastQC (version 0.11.5)