FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27076010
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG194300.023.26011550-51
AAAGGGG527150.021.59489480-81
GGGGAAT248800.018.52292362-63
AAAAAGG671450.017.69699578-79
AAAAGGG542550.015.24132878-79
GGAATTA171200.015.14515464-65
GGGAAAA430800.015.00911222-23
AAGGGGG553750.014.81687680-81
GAATTAA204750.014.76282166-67
AGGGGGG842900.014.6940682-83
GGGAATT205800.014.57178664-65
GGGGGAG557150.014.4689473
ATAGGGG126950.014.350224548-49
GGGGGGT694100.013.97875414-15
GGGGGGA673700.013.4244192
TAAAAAA932600.013.34144170-71
TATAGGG110850.013.04901248-49
GGGGGAA507800.011.93692662-63
GGGTTGG278450.011.7424616-17
AAAAAAG882300.011.57408776-77
Produced by FastQC (version 0.11.5)