FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28128883
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG215850.025.93338650-51
AAAGGGG542750.022.67119480-81
GGGGAAT264050.019.43119262-63
AAAAAGG708550.018.35837778-79
GGGGGAG563850.017.464933
GGGGGGT639200.017.01960814-15
GGGAAAA496950.016.69230722-23
GGAATTA177450.016.65877564-65
AGGGGGG838500.015.89210982-83
GAATTAA224000.015.762566-67
GGGGGGA712450.015.6075832
AAAAGGG566200.015.12864778-79
AAGGGGG565950.015.03848680-81
GGGAATT211600.015.03622864-65
TTTAGGG188250.014.98928948-49
TTAGGGG189600.014.61948648-49
TATAGGG116050.013.897148-49
TAAAAAA993000.013.5461570-71
GGGGAGA400150.013.2979144
GGGTTGG307700.012.98925616-17
Produced by FastQC (version 0.11.5)