FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25703308
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG166400.021.83659450-51
AAAAGGG467250.019.23248178-79
AAGGGGG472650.019.07742180-81
AAAGGGG451100.017.20467480-81
TAAAAAA836050.013.76043770-71
AAAAAAG786600.013.70661576-77
AAAAAGG593250.013.69496878-79
AGGGGGG702150.013.67702982-83
GGGTTAA91600.013.58311366-67
GGGGAAT202800.012.58570162-63
TTTAGGG140300.011.85041748-49
TTAGGGG147900.011.81960448-49
GGGGTTA111900.011.543138564-65
GGGAAAA345700.010.98717122-23
GTTAAAA220900.010.96421968-69
CGCGGGT57350.010.8529811
GGGGGGT739500.010.69117814-15
GGGGGAT252900.010.57125362-63
GGAATTA144150.010.24544464-65
GAATTAA169550.010.15321866-67
Produced by FastQC (version 0.11.5)