FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n02_AHPm101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n02_AHPm101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24062625
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG174950.026.79704550-51
AAAGGGG434150.021.60605880-81
GGGGAAT213300.018.32232962-63
TATAGGG101600.017.62660848-49
AAAAAGG568550.017.4594978-79
GGGGGGT496200.016.51734514-15
GGGAAAA393700.016.1882422-23
ATAGGGG113700.016.04323248-49
TTTAGGG152950.015.855041548-49
GGGGGAG457650.015.3308873
GAATTAA180400.015.07064766-67
AGGGGGG670700.014.9945682-83
AAAAGGG453350.014.92034678-79
TTAGGGG155400.014.71859748-49
GGGGGGA549350.014.4656562
AAGGGGG459400.014.40304480-81
GGAATTA152250.014.34784664-65
GGGAATT178150.013.44811864-65
TTATAGG120100.013.01354746-47
TAAAAAA793700.012.56203870-71