FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21591605
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3093171.4325799309500151No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT347900.16112743818720285TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC256050.054.0737538
TCGGAAG273500.050.209163
CGGAAGA278100.049.801854
ATCGGAA279800.049.6542472
GATCGGA279000.048.2981221
TCTCGTA104850.036.22992742-43
CGTCTGA189200.035.5606816-17
ACGTCTG189750.035.26801314-15
GAGCACA399950.035.0085149
ACACGTC195250.034.96289412-13
CACGTCT193850.034.8390214-15
CTCGTAT103800.034.28411544-45
ATGCCGT120150.034.0586248-49
CACACGT204100.033.71314612-13
CGTATCT37800.033.22667338-39
AAGAGCA431400.033.0584757
TATCTCG40850.032.8524340-41
GCACACG215900.031.78303710-11
ATCTCGT111350.031.50723342-43
GTCACGA44100.031.40593528-29
Produced by FastQC (version 0.11.5)