FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24272938
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2233180.9200287167544365TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG260300.043.06984344-45
CGTATGC263650.042.88403346-47
TCGTATC262000.042.39579838-39
CTCGTAT264300.042.14829344-45
TATGCCG269200.041.95577248-49
CGTATCT263300.041.72645638-39
TCGGAAG555950.041.1748163
GAGCACA562100.041.1373869
ATCGGAA556500.041.0926552
ATTCGTA277900.041.02173236-37
TATCTCG270100.040.6044840-41
AGAGCAC566500.040.68
GATCGGA562900.040.5580641
TTCGTAT279150.040.29364836-37
GTCACGA283950.040.18743528-29
CGGAAGA573600.040.0064164
AATTCGT287450.039.53954734-35
ACGAATT291000.039.37210532-33
TCTCGTA280250.039.2116342-43
CACGAAT291850.039.0912830-31
Produced by FastQC (version 0.11.5)