FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24986147
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2304080.9221429778668956TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG269600.043.4268744-45
CGTATGC273800.043.04832546-47
TCGTATC271050.042.47979738-39
CTCGTAT274300.042.370944-45
TATGCCG280050.042.09553548-49
CGTATCT273700.041.74743338-39
TATCTCG278600.041.01151740-41
ATTCGTA290850.040.68298336-37
TTCGTAT290850.040.10331336-37
GAGCACA608650.039.7131969
GTCACGA299750.039.70018828-29
AATTCGT299650.039.42801334-35
TCTCGTA292050.039.18103842-43
ACGAATT304100.039.1280532-33
ATCGGAA608200.039.0579342
AGAGCAC617200.038.9400148
CACGAAT306000.038.8039130-31
TCGGAAG617000.038.5922743
GAATTCG308450.038.5879534-35
CGAATTC310050.038.26231432-33
Produced by FastQC (version 0.11.5)