FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24641312
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2183970.8863042682142899TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG262050.043.4074844-45
CGTATGC266400.042.85158546-47
TCGTATC264300.042.31566238-39
CTCGTAT265650.042.2378244-45
TATGCCG271000.042.10624748-49
CGTATCT267950.041.57969738-39
TATCTCG271550.041.0453840-41
ATCGGAA571000.040.671482
TTCGTAT282450.040.318836-37
ATTCGTA287050.040.0366636-37
GAGCACA588900.039.8208859
TCGGAAG585600.039.7946323
GTCACGA291550.039.77494428-29
ACGAATT293450.039.4792532-33
GATCGGA587700.039.4109881
AGAGCAC595400.039.338328
AATTCGT294300.039.09496734-35
TCTCGTA286300.038.94762442-43
CACGAAT298750.038.72776430-31
CGGAAGA602400.038.6841934
Produced by FastQC (version 0.11.5)