FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26461884
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2399000.9065869988697706TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC288100.043.3677846-47
TCGTATG286950.043.36632544-45
CTCGTAT289850.042.45696344-45
TCGTATC288300.042.4252638-39
TATGCCG295000.042.2085848-49
CGTATCT290400.041.79133238-39
TATCTCG297200.040.88942340-41
ATTCGTA312100.040.27876336-37
TTCGTAT310050.040.00131636-37
TCTCGTA304500.039.92703242-43
AATTCGT318100.039.45516634-35
CACGAAT319950.039.37535530-31
GAGCACA646250.039.2543839
GTCACGA321250.039.21623628-29
ACGAATT323650.039.13335432-33
TCGGAAG648000.038.936523
AGTCACG327850.038.56434628-29
ATCTCGT318850.038.2269742-43
CGGAAGA666700.037.9578974
CGAATTC335000.037.69410732-33
Produced by FastQC (version 0.11.5)