FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26283038
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2625570.9989598614893758TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG609700.044.816253
CGTATGC309400.043.65603646-47
TCGTATG308350.043.64148344-45
GATCGGA627350.043.497451
CGGAAGA635700.043.0421074
ATCGGAA636750.042.920992
TCGTATC311050.042.6890438-39
TATGCCG316900.042.59251848-49
CTCGTAT312000.042.59017644-45
GAGCACA643250.042.5809489
CGTATCT313550.042.00024438-39
TATCTCG318100.041.4304140-41
AGAGCAC662750.041.3710758
ATTCGTA332100.040.90487336-37
TTCGTAT330450.040.79294636-37
TCTCGTA324250.040.67703242-43
AATTCGT338100.040.23044234-35
CACGAAT339750.040.15297730-31
ACGAATT344500.039.62977232-33
ATCTCGT334200.039.52996442-43
Produced by FastQC (version 0.11.5)