FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25473530
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2152800.8451125540904618TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG264100.043.12396244-45
CGTATGC268150.042.46490546-47
ATCGGAA551800.041.9330372
TATGCCG272300.041.91346448-49
TCGTATC267900.041.8523238-39
CTCGTAT269450.041.7915244-45
GATCGGA559250.041.274281
CGTATCT271650.041.21336438-39
TCGGAAG566900.040.9073333
TATCTCG272950.040.88520440-41
ATTCGTA288300.039.83968736-37
TTCGTAT286000.039.76984836-37
GAGCACA589800.039.6402179
TCTCGTA284200.039.25946842-43
AGAGCAC593900.039.2386678
CACGAAT294150.039.22889330-31
CGGAAGA594800.039.051754
AATTCGT298150.038.68668734-35
ACGAATT299300.038.55763632-33
GTCACGA300600.038.49828328-29
Produced by FastQC (version 0.11.5)