FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26180516
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1770610.6763082897220208TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG218000.041.14761444-45
TATGCCG219900.041.0845948-49
CGTATGC220550.040.9747546-47
CTCGTAT221450.040.20612344-45
TCGTATC226200.039.21631638-39
TATCTCG227000.038.7291140-41
CGTATCT227700.038.48859838-39
TCTCGTA236250.037.14138442-43
ATTCGTA249450.036.44882236-37
TTCGTAT247900.036.05410436-37
ATCTCGT253600.034.9563942-43
AATTCGT262050.034.62864334-35
CACGAAT269450.033.74212330-31
ATGCCGT267850.033.69421848-49
GTATGCC272600.033.19465346-47
ACGAATT277650.033.04742432-33
GTCACGA278300.032.68648528-29
GAATTCG283350.032.38584534-35
CGAATTC285350.031.94766632-33
CCGTCTT283900.031.86330852-53
Produced by FastQC (version 0.11.5)