FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26533433
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3933731.4825559888914488TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG453950.044.61413644-45
CGTATGC458050.044.30399346-47
TATGCCG461650.043.84036348-49
CTCGTAT450800.043.66081244-45
TCTCGTA467900.042.02609342-43
GAGCACA996450.041.7994429
AATCTCG475250.041.3095740-41
AGAGCAC1020600.040.77788
ATCTCGT482200.040.5974642-43
GTATGCC511900.039.75478446-47
ATGCCGT510300.039.74461448-49
CCGTCTT517100.039.03660252-53
GCCGTCT518550.038.95607450-51
CGGAAGA1102000.037.6881834
TCGGAAG1105600.037.4887433
AAGAGCA1125750.037.0027127
TGCCGTC549450.036.8777150-51
ATCGGAA1140900.036.313482
CGTCTTC558100.036.20289652-53
GATCGGA1176300.035.156151
Produced by FastQC (version 0.11.5)