FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25228719
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3863851.531528414106162TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA875050.046.8387229
AGAGCAC898850.045.667178
TCGTATG448050.044.676544-45
CGTATGC451700.044.43258346-47
TATGCCG455450.043.9520948-49
TCGGAAG931900.043.920493
CTCGTAT444450.043.7926544-45
CGGAAGA942500.043.4917534
ATCGGAA954450.042.819012
TCTCGTA459050.042.3599442-43
AATCTCG463550.041.91311640-41
GATCGGA975250.041.8179471
AAGAGCA984550.041.745127
ATCTCGT472000.041.0366542-43
GTATGCC502150.040.0679146-47
ATGCCGT501500.040.0299348-49
CCGTCTT508300.039.2077652-53
GCCGTCT510150.039.14138450-51
GAAGAGC1061000.038.772986
TGCCGTC536250.037.4091450-51
Produced by FastQC (version 0.11.5)