FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34102660
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT4997171.4653314433536857TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1133500.046.5170449
AGAGCAC1155100.045.622538
TCGTATG582300.044.27511244-45
CGTATGC585150.044.10143746-47
TATGCCG588900.043.82441348-49
CTCGTAT577750.043.5297944-45
ATCGGAA1213150.043.2459032
TCGGAAG1215800.043.1856423
CGGAAGA1228550.042.7790224
TCTCGTA599700.041.9842842-43
GATCGGA1254400.041.715341
AATCTCG604550.041.59120640-41
ATCTCGT617300.040.64484442-43
AAGAGCA1298000.040.603497
GTATGCC653950.039.58884446-47
ATGCCGT655250.039.41943448-49
GCCGTCT666000.038.61147350-51
CCGTCTT668250.038.50861752-53
GAAGAGC1404300.037.7158976
TGCCGTC705000.036.603650-51
Produced by FastQC (version 0.11.5)