FastQCFastQC Report
Thu 27 Jun 2019
HJ75TBGXB_n01_AHPm105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJ75TBGXB_n01_AHPm105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27878620
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3571581.2811179319492858TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA831050.045.3269739
AGAGCAC836500.044.9749078
TCGTATG417250.044.09488744-45
ATCGGAA854100.043.788632
CGTATGC421900.043.70573846-47
TATGCCG424450.043.44907448-49
TCGGAAG863450.043.4010283
CTCGTAT416400.043.12350544-45
CGGAAGA880150.042.625854
GATCGGA877900.042.4926071
TCTCGTA431200.041.59065242-43
AATCTCG438400.040.8538240-41
ATCTCGT446550.040.086542-43
AAGAGCA953100.039.5873457
GTATGCC476950.038.740946-47
ATGCCGT479550.038.4914948-49
CCGTCTT485000.037.69497352-53
GCCGTCT487550.037.50390650-51
GAAGAGC1034300.036.539126
TGCCGTC518750.035.48644650-51
Produced by FastQC (version 0.11.5)